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Inhibition by galanin and by high K + of human basophil histamine release triggered by calcium ionophores but not responses induced by anti‐IgE, chemotactic peptide or phorbol ester
Author(s) -
Bergstrand Håkan,
Lundquist Britta,
Ahrén Bo
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb09798.x
Subject(s) - histamine , ionomycin , basophil , galanin , chemistry , pertussis toxin , calcium , ionophore , phorbol , n formylmethionine leucyl phenylalanine , medicine , pharmacology , biochemistry , endocrinology , chemotaxis , immunoglobulin e , neuropeptide , protein kinase c , biology , immunology , receptor , g protein , signal transduction , antibody , organic chemistry
1 Galanin concentration‐dependently blocked human leukocyte histamine release triggered by the calcium ionophores A23187 and ionomycin. Almost complete inhibition of release was recorded at 410 n m whereas 41 n m galanin mediated close to 50% inhibition of responses. 2 Pretreatment of the cells with pertussis toxin did not influence the inhibitory effects of galanin. 3 Leukocyte responses triggered by anti‐IgE, the chemotactic peptide formyl‐methionyl‐leucyl‐phenylalanine (FMLP) or 4β‐phorbol 12‐myristate 13‐acetate (PMA) were not affected by galanin. 4 Ionophore‐induced basophil histamine release, but not responses triggered by anti‐IgE, FMLP or PMA, was inhibited when cells were challenged in medium containing high potassium (120 m m ). 5 We conclude that galanin and a depolarizing medium selectively inhibit ionophore‐induced basophil histamine release.