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Platelet‐leukocyte interaction in adhesion to endothelial cells induced by platelet‐activating factor in vitro
Author(s) -
Hirafuji Masahiko,
Shinoda Hisashi
Publication year - 1991
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1991.tb09789.x
Subject(s) - platelet , platelet activating factor , adhesion , endothelial stem cell , cell–cell interaction , in vitro , chemistry , cell adhesion , platelet adhesiveness , microbiology and biotechnology , immunology , biology , biochemistry , platelet aggregation , organic chemistry
1 Platelet‐activating factor (PAF, 10 n m ) did not induce platelet adhesion to endothelial cells cultured in monolayer but it induced their adhesion to protein‐coated plastic. However, PAF induced a marked platelet adhesion to endothelial cells when polymorphonuclear leukocytes (PMNs) were present. Lyso‐PAF had no effect. 2 Phase‐contrast microscopic examination showed that single platelets rather than their aggregates adhered to the endothelial cell surface around aggregating and adhering PMNs. 3 Significant platelet adhesion was induced by PAF at concentrations higher than 0.01 n m with the maximal response at 10 n m . Platelet adhesion occurred within minutes after PAF addition, reaching a maximum approximately after 30 min. Platelet adhesion also occurred significantly at a PMN: platelet ratio of 1:800, and linearly up to 1:50. 4 The PAF‐induced platelet adhesion was suppressed by three structurally unrelated PAF antagonists, WEB 2086, ONO 6240 and BN 52021, in a concentration‐dependent manner. 5 PAF also increased PMN adhesion to endothelial cell monolayers, which was further augmented by the presence of platelets. 6 The present study demonstrates that PAF induces platelet adhesion to endothelial cells in vitro when PMNs are present and that there is a close interaction between platelets and PMNs in their adhesion to endothelial cells. The present study further suggests that PMNs could play a central role in platelet adhesion to vascular endothlium in certain pathological conditions.