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Eosinophil accumulation in pulmonary airways of guinea‐pigs induced by exposure to an aerosol of platelet‐activating factor: effect of anti‐asthma drugs
Author(s) -
Sanjar S.,
Aoki S.,
Boubekeur K.,
Chapman I.D.,
Smith D.,
Kings M.A.,
Morley J.
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb14692.x
Subject(s) - platelet activating factor , eosinophil , bronchoalveolar lavage , salbutamol , inhalation , medicine , eosinophilia , methacholine , immunology , pharmacology , mepyramine , lung , asthma , anesthesia , antagonist , respiratory disease , receptor
1 Exposure of guinea‐pigs to aerosols of platelet activating factor (PAF) (0.01 to 100 μg ml −1 ) induced a dose‐dependent increased incidence of eosinophils in bronchoalveolar lavage fluid (BAL) at 48 h. Total leucocyte numbers and the percentages of lymphocytes and neutrophils were unchanged in BAL fluid. 2 Increased numbers of eosinophils were detected in BAL 1 h after exposure to PAF but eosinophilia was not maximal until 48 h. One week after exposure to PAF, the percentage of eosinophils in BAL was within the normal range. 3 Depletion of circulating platelets or neutrophils by intravenous injection of specific antisera did not modify accumulation of eosinophils in the airway lumen following inhalation of PAF (10 μg ml −1 ). 4 PAF‐induced pulmonary airway eosinophil accumulation was inhibited by treatment with SDZ 64–412, a selective PAF‐antagonist, whether the compound was administered before, or 30 min after, inhalation of PAF. 5 Pulmonary airway eosinophil accumulation due to inhaled PAF (10 μg ml −1 ) was inhibited by prior treatment with aminophylline, cromoglycate, ketotifen, dexamethasone and AH 21–132. 6 Pulmonary airway eosinophil accumulation due to inhaled PAF (10 μg ml −1 ) was not inhibited by prior treatment with indomethacin, salbutamol or mepyramine.