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Action of calcitonin gene‐related peptide upon bovine vascular endothelial and smooth muscle cells grown in isolation and co‐culture
Author(s) -
Crossman D.C.,
Dashwood M.R.,
Brain S.D.,
McEwan J.,
Pearson J.D.
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb14656.x
Subject(s) - calcitonin gene related peptide , receptor , calcitonin , medicine , bradykinin , adenylate kinase , cyclase , endocrinology , agonist , vascular smooth muscle , chemistry , biology , smooth muscle , neuropeptide
1 Bovine aortic endothelial cells (BAE) and smooth muscle cells (BASM) were grown separately and in co‐culture. 2 Calcitonin gene‐related peptide (CGRP) caused dose‐dependent activation of adenylate cyclase in each cell type when grown in isolation. The concentration of CGRP causing half‐maximal activation in BAE and BASM was 200 n m and 310 n m , respectively. 3 In cells grown in co‐culture exposure to bradykinin produced dose‐dependent elevations in cyclic GMP content which were maximal 1 min after application of the agonist. 4 CGRP (1 n m −1 μ m ) did not stimulate a rise in cyclic GMP in co‐cultures. 5 Displaceable CGRP binding was identified throughout the wall of the bovine aorta. 6 We conclude that CGRP receptors coupled to adenylate cyclase are present on BAE and BASM, but there is no coupling of these receptors to the release of any agent (such as endothelium‐derived relaxing factor) that activates guanylate cyclase.