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Inhibition of neutrophil and eosinophil induced chemotaxis by nedocromil sodium and sodium cromoglycate
Author(s) -
Bruijnzeel P.L.B.,
Warringa R.A.J.,
Kok P.T.M.,
Kreukniet J.
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb13009.x
Subject(s) - nedocromil sodium , chemotaxis , nedocromil , chemistry , leukotriene b4 , cromolyn sodium , eosinophil , pharmacology , platelet activating factor , zymosan , immunology , inflammation , biochemistry , in vitro , medicine , asthma , receptor , respiratory disease , alternative medicine , pathology , lung , placebo
1 Neutrophils and eosinophils infiltrate the airways in association with the allergen‐induced late phase asthmatic reaction. Mobilization of these cells takes place via lipid‐like and protein‐like chemotactic factors. In this study platelet‐activating factor (PAF), leukotriene B 4 (LTB 4 ), zymosan‐activated serum (ZAS) and N‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP) were used as illustrative examples of both groups. Chemotaxis was studied in human neutrophils and eosinophils. The inhibitory effects of nedocromil sodium and sodium cromoglycate were evaluated. 2 All chemotactic factors tested attracted neutrophils with the following rank order of activity: ZAS > PAF ≡ FMLP ≡ LTB 4 . Eosinophils were only mobilized by PAF, LTB 4 and ZAS with the following rank order of activity: ZAS > PAF > LTB 4 . 3 Nedocromil sodium and sodium cromoglycate were equally active as the PAF antagonist BN 52021 in inhibiting the PAF‐induced chemotaxis of neutrophils (IC 50 & 10 −8 m ). Both drugs were also equally active in inhibiting the chemotaxis of neutrophils induced by ZAS (IC 50 ≅ 10 −7 −10 −6 m ), FMLP(IC 50 ≅ 10 −7 m ) and LTB 4 (IC 50 ≅ 10 −6 m ). 4 Nedocromil sodium significantly inhibited the chemotaxis of eosinophils induced by PAF (IC 50 ≅ 10 −6 m ) and LTB 4 (IC 50 ≅ 10 −7 m ). The inhibitory potency of BN 52021 was similar to that of nedocromil sodium on the PAF‐induced chemotaxis of eosinophils. Sodium cromoglycate was incapable of eliciting significant inhibition of these chemotactic responses. However, sodium cromoglycate significantly inhibited the chemotaxis of eosinophils induced by ZAS (IC 50 ≅ 10 −7 m ), whereas nedocromil sodium was ineffective.