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The role of the epithelium in modulating the responses of guinea‐pig trachea induced by bradykinin in vitro
Author(s) -
Bramley Andrew M.,
Samhoun Marwa N.,
Piper Priscilla J.
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb13003.x
Subject(s) - bradykinin , epithelium , endocrinology , contraction (grammar) , guinea pig , medicine , acetylcholine , prostaglandin e2 , chemistry , stimulation , biology , genetics , receptor
1 The effect of removing the epithelium on the responses of the guinea‐pig isolated trachea (GPT) to bradykinin (BK) and prostaglandin E 2 (PGE 2 ) was investigated. 2 BK (3 pmol–10 nmol) induced dose‐related relaxations of the intact (with epithelium), and contracted the rubbed (without epithelium) preparation of GPT. Similar responses were also obtained with PGE 2 (0.3–3.0 nmol). 3 Indomethacin (1.4 μ m ) modified the BK‐induced response of intact GPT, from a relaxation to a contraction, but inhibited the BK‐induced contraction of the rubbed GPT. 4 There was a significant increase in PGE 2 release from the intact GPT following stimulation with BK. 5 Removal of the epithelium from the GPT significantly reduced both basal and BK‐induced generation of PGE 2 . 6 The induction of tone in the rubbed GPT by addition of acetylcholine (ACh) caused BK and PGE 2 (0.3 nmol–3 nmol) to produce relaxations of the tissue. 7 Salbutamol (10 −8 m ‐10 −6 m ) reduced the relaxations induced by BK on intact GPT, in a concentration‐dependent manner. 8 These results suggest that both tone and an epithelial‐dependent cyclo‐oxygenase mechanism are important in modulating BK‐induced responses of GPT.