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The effects of substance P analogues on the scratching, biting and licking response induced by intrathecal injection of N‐methyl‐ d ‐aspartate in mice
Author(s) -
Sakurada Tsukasa,
Manome Yoichi,
TanNo Koichi,
Sakurada Shinobu,
Kisara Kensuke
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb12706.x
Subject(s) - nmda receptor , licking , scratching , substance p , biting , pharmacology , chemistry , 2 amino 5 phosphonovalerate , receptor , desensitization (medicine) , antagonist , receptor antagonist , medicine , endocrinology , biology , excitatory amino acid antagonists , neuropeptide , biochemistry , ecology , physics , acoustics
1 Intrathecal (i.t.) administration of N‐methyl‐ d ‐aspartate (NMDA) elicited a dose‐dependent behavioural response consisting of licking, biting and scratching in mice. 2 Repeated i.t. injections of 0.4 nmol NMDA, at 5 min intervals, resulted in the rapid development of desensitization to this NMDA‐induced behavioural phenomenon. 3 The NMDA‐induced response was dose‐dependently inhibited by the simultaneous injection of a selective NMDA‐receptor antagonist, d ‐2‐amino‐5‐phosphonovaleric acid. 4 The substance P (SP) analogues [ d ‐Pro 2 , d ‐Trp 7,9 ] SP and [ d ‐Arg 1 , d ‐Trp 7,9 , Leu 11 ] SP (spantide) inhibited NMDA‐induced behavioural responses in a dose‐dependent manner. However, [ d ‐Phe 7 , d ‐His 9 ] SP (6–11), a SP analogue selective for neurokinin 1 (NK 1 ) receptors, failed to inhibit NMDA‐induced responses even at a dose of 4.0 nmol. 5 These results indicate that NMDA‐induced behavioural responses are mainly mediated through NMDA receptors without affecting NK 1 receptors in the spinal cord.