Premium
The effects of acute and chronic lithium treatment on pilocarpine‐stimulated phosphoinositide hydrolysis in mouse brain in vivo
Author(s) -
Whitworth P.,
Heal D.J.,
Kendall D.A.
Publication year - 1990
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1990.tb12085.x
Subject(s) - pilocarpine , in vivo , inositol , lithium chloride , chemistry , atropine , endocrinology , medicine , saline , lithium (medication) , inositol phosphate , pharmacology , biochemistry , biology , epilepsy , receptor , microbiology and biotechnology , organic chemistry , neuroscience
1 Measurements were made of the in vivo formation of inositol phosphates in the brains of C57/B1/601a mice treated acutely or chronically with lithium chloride (LiCl). 2 A single injection of LiCl (10 m Equiv kg −1 , s.c.) 18 h before death increased the accumulation of [ 3 H]‐inositol phosphates ([ 3 H]‐Ins P's) in the brains of mice injected i.c.v. with [ 3 H]‐myo‐inositol 24 h previously. 3 Pilocarpine (200 mg kg −1 , i.p.) injected 15 min before death further enhanced the formation of [ 3 H]‐Ins P's in the brains of LiCl‐treated, but not saline‐treated, mice. The enhancement due to pilocarpine was abolished by injection of atropine sulphate (10 mg kg −1 , i.p.) 10 min earlier. 4 Chronic (14 days) LiCl feeding produced an accumulation of [ 3 H]‐Ins P's significantly less than that due to a single injection of LiCl, but the response to pilocarpine was markedly greater in mice chronically fed with LiCl when compared with mice acutely injected with LiCl. 5 Mass measurements of endogenous inositol 1,4,5 triphosphate revealed increases due to pilocarpine and chronic LiCl feeding alone. A combination of the two treatments produced levels greater than either alone. 6 These results demonstrate that LiCl treatment enhances both basal and pilocarpine‐stimulated inositol phospholipid hydrolysis in vivo and this might be relevant to its therapeutic effects.