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Cholera and pertussis toxins amplify prostacyclin synthesis in aortic smooth muscle cells
Author(s) -
Demolle D.,
Boeynaems J.M.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb14597.x
Subject(s) - prostacyclin , pertussis toxin , cholera toxin , arachidonic acid , phorbol , guanosine , medicine , chemistry , endocrinology , prostaglandin , biology , biochemistry , protein kinase c , g protein , receptor , signal transduction , enzyme
Pretreatment of bovine aortic smooth muscle cells in culture with pertussis toxin (PT) or cholera toxin (CT) potentiated the synthesis of prostacyclin (PGI 2 ) induced by 5‐hydroxytryptamine (5‐HT) and phorbol‐12‐myristate, 13‐acetate (PMA). The production of PGI 2 by explants from the bovine aortic media was also synergistically stimulated by 5‐HT and CT, whereas PT was inactive. These data are consistent with the hypothesis that guanosine 5′‐triphosphate binding proteins are directly involved in the control of phospholipases which release free arachidonic acid for prostaglandin synthesis.