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Endothelin stimulates short circuit current in a cultured epithelium
Author(s) -
Wong P.Y.D.,
Fu W.O.,
Huang S.J.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb12664.x
Subject(s) - endocrinology , medicine , endothelin 1 , angiotensin ii , endothelin receptor , epithelium , chemistry , prostacyclin , adenosine , stimulation , biology , receptor , genetics , blood pressure
1 Endothelin, a novel potent vasoconstrictor peptide produced by vascular endothelial cells stimulated anion secretion by a cultured secretory epithelium derived from the rat epididymis as measured by changes in short‐circuit current (SCC). 2 Stimulation of the SCC was observed when endothelin was added to the basolateral or the apical side of the epithelium. The response to basolateral application was greater than that to apical application. The EC 50 values were found to be 1.3 nM and 3.0 nM for basolateral and apical application, respectively. These values were about half an order to one order of magnitude higher than that required for its vasoconstrictor action. 3 The stimulation of SCC by endothelin was accompanied by a rise in the intracellular adenosine 3′:5′‐cyclic monophosphate (cyclic AMP) content in epididymal monolayers. 4 The effect of endothelin on SCC was mediated through an increase in prostaglandin synthesis by the tissues and was not blocked by an antagonist of angiotensin II (Sar 1 Ile 8 angiotensin II) or of adrenaline (propranolol). 5 It is speculated that endogenous endothelin plays an important role in the control of water and electrolyte transport in the epididymis.