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Superoxide anions enhance platelet adhesion and aggregation
Author(s) -
Salvemini Daniela,
Nucci Gilberto,
Sneddon John M.,
Vane John R.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb12572.x
Subject(s) - chemistry , catalase , superoxide dismutase , hydrogen peroxide , radical , thrombin , superoxide , adhesion , platelet , pyrogallol , biochemistry , reactive oxygen species , mannitol , platelet adhesiveness , biophysics , platelet aggregation , oxidative stress , enzyme , immunology , organic chemistry , biology
1 Superoxide dismutase (SOD, 60 u ml −1 ) or ferricytochrome c (70 μ m ) significantly inhibited thrombin‐stimulated platelet adhesion to gelatin‐coated plastic, whereas catalase (1000 u ml −1 ) or mannitol (1 m m ) had no effect. 2 The platelet aggregation induced by low concentrations of thrombin (causing less than 45% maximal change in light transmission) was inhibited by SOD. Catalase or mannitol had no effect on platelet aggregation. 3 Pyrogallol (an O 2 − generator) enhanced both platelet adhesion to gelatin‐coated plastic and platelet aggregation induced by thrombin; this enhancement was neutralized by SOD. 4 These results indicate that O 2 − increase both platelet adhesion and aggregation, whereas other free radicals such as hydrogen peroxide or hydroxyl radicals are not involved.