Premium
Pertussis toxin prevents the inhibitory effect of adenosine and unmasks adenosine‐induced excitation of mammalian motor nerve endings
Author(s) -
Silinsky E.M.,
Solsona C.,
Hirsh J.K.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb11918.x
Subject(s) - adenosine , inhibitory postsynaptic potential , pertussis toxin , acetylcholine , free nerve ending , chemistry , motor nerve , neuromuscular junction , medicine , endocrinology , pharmacology , g protein , neuroscience , biology , biochemistry , receptor
Pertussis toxin (PTX), which blocks certain classes of guanine nucleotide binding proteins (G proteins), consistently blocked the inhibitory effects of adenosine (100 μ m ‐250 μ m ) on quantal acetylcholine (ACh) secretion in rat phrenic nerve hemidiaphragm preparations. PTX pretreatment also highlighted long‐lasting increases in evoked ACh release elicited by adenosine. The results suggest that specific G proteins are involved in mediating the inhibitory effects of adenosine at motor nerve endings.