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The effect of glucocorticosteroids on in vitro motility of the ureter of the sheep
Author(s) -
AngeloKhattar Maria,
Thulesius Olav,
Cherian Thomas
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb11849.x
Subject(s) - dexamethasone , methylprednisolone , cycloheximide , hydrocortisone , endocrinology , medicine , pharmacology , steroid , motility , potency , corticosteroid , in vitro , chemistry , biology , protein biosynthesis , biochemistry , hormone , genetics
1 The effects of three glucocorticosteroids, hydrocortisone, methylprednisolone and dexamethasone, as well as two non‐steroidal anti‐inflammatory agents (NSAIDs) indomethacin and diclofenac sodium were tested in vitro on the spontaneously rhythmic contracting ureteral preparation of the sheep. 2 The NSAIDs and the steroids methylprednisolone (10 −7 ‐10 −4 m ) and dexamethasone (10 −8 ‐10 −4 m ), dose‐dependently inhibited ureteral motility. Hydrocortisone caused a cessation of spontaneous contractions only at the high concentration of 10 −4 m . 3 Pretreatment of ureteral strips with the protein synthesis inhibitor cycloheximide (10 −6 m ) abolished the inhibitory action of the corticosteroids on peristalsis, consistent with the suggestion that the action of steroids on the ureter is mediated via the synthesis of the anti‐phospholipase protein, lipocortin. 4 The potency of the steroids in descending order was found to be dexamethasone > methylprednisolone > hydrocortisone.