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Evidence for an intracellular action of platelet‐activating factor in bovine cultured aortic endothelial cells
Author(s) -
Stewart Alastair G.,
Dubbin Philip N.,
Harris Trudi,
Dusting Gregory J.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb11845.x
Subject(s) - bradykinin , prostacyclin , platelet activating factor , intracellular , platelet , chemistry , receptor , microbiology and biotechnology , endothelial stem cell , cell culture , endocrinology , medicine , biochemistry , biology , in vitro , genetics
Bovine culture endothelial cells (BAECs) generate platelet‐activating factor (Paf) following activation by bradykinin (Bk 0.1 n m ), the ionophore, A23187 (3 μ m ), and ATP (10 μ m ), but Paf is not released from the cells. These stimuli also elicit generation of prostacyclin (PGI 2 ). The specific and competitive Paf receptor antagonists, WEB 2086 (0.1–1.0 μ m ) and CV 6209 (0.01–0.1 μ m ), inhibited Bk‐, A23187‐ and, to a lesser extent, ATP‐induced PGI 2 generation but had no effect on basal PGI 2 generation. These data suggest a role for intracellular Paf in signal transduction.