Premium
Role of endoperoxides in arachidonic acid‐induced vasoconstriction in the isolated perfused kidney of the rat
Author(s) -
Quilley J.,
McGiff J.C.,
Nasjletti A.
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb11790.x
Subject(s) - arachidonic acid , vasoconstriction , thromboxane a2 , chemistry , thromboxane , kidney , prostaglandin h2 , prostaglandin , endocrinology , medicine , angiotensin ii , pharmacology , receptor , biochemistry , platelet , biology , enzyme
1 Administration of arachidonic acid caused dose‐dependent vasoconstriction in the isolated rat kidney perfused in situ with Krebs‐Henseleit solution. 2 Inhibition of cyclo‐oxygenase with indomethacin or meclofenamate reduced the renal vasoconstrictor effect of arachidonic acid. 3 The renal vasoconstrictor effect of arachidonic acid was unaffected by CGS‐13080 at concentrations that effectively reduced thromboxane A 2 (TxA 2 ) synthesis by platelets and the kidney. 4 The endoperoxide/TxA 2 receptor antagonist, SQ 29,548, abolished the renal vasoconstrictor effect of arachidonic acid and of U46619, an endoperoxide analogue. In contrast, SQ 29,548 did not affect the renal vasoconstrictor response to angiotensin II, prostaglandin E 2 or F 2α . 5 These data suggest that the vasoconstrictor effect of arachidonic acid in the isolated kidney of the rat is mediated by its metabolites, including the prostaglandin endoperoxides.