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Release of 5‐hydroxytryptamine, dopamine and noradrenaline in the rat vas deferens in the presence of compound 48/80, veratridine or K+
Author(s) -
Celuch Stella Maris,
Sloley B. Duff
Publication year - 1989
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1989.tb11786.x
Subject(s) - veratridine , vas deferens , dopamine , histamine , neurotransmitter , chemistry , medicine , depolarization , endocrinology , norepinephrine , pharmacology , biology , sodium , central nervous system , sodium channel , organic chemistry
1 The release of 5‐hydroxytryptamine (5‐HT), dopamine and noradrenaline (NA) from the rat isolated vas deferens was estimated by high performance liquid chromatography with electrochemical detection. 2 Compound 48/80, which is known to release 5‐HT and histamine from mast cells, induced a concentration‐related, rapid overflow of 5‐HT from the rat isolated vas deferens, releasing about 50% of the 5‐HT present in the tissue at a concentration of 300 μg ml −1 . The drug did not induce the release of either dopamine or NA. 3 The depolarizing agents veratridine (10 μ m ) or K + (100m m ) induced the release of 5–10% of the 5‐HT present in the vas deferens, but did not evoke the overflow of 5‐HT from isolated mast cells. 4 Veratridine induced the release of a greater proportion of the dopamine than of the NA contained in the vas deferens (41.0 ± 5.5 vs 17.9 ± 2.0%, after 20 min of exposure to the drug, n = 6). In contrast, K + evoked the release of both amines equally. 5 It is concluded that in the rat vas deferens 5‐HT is stored in mast cells and also in cells responsive to depolarizing stimuli. It is suggested that 5‐HT could be a neuromodulator or a neurotransmitter in this organ. Veratridine and K + induce the release of dopamine in addition to NA.