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Contrasting in vitro lymphocyte chemotactic activity of the hydroxyl enantiomers of 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid
Author(s) -
Bacon K.B.,
Camp R.D.R.,
Cunningham F.M.,
Woollard P.M.
Publication year - 1988
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1988.tb11727.x
Subject(s) - chemotaxis , leukotriene b4 , zymosan , lymphocyte , in vitro , chemistry , immunology , biochemistry , microbiology and biotechnology , pharmacology , biology , inflammation , receptor
1 The chemotactic activity of 12(R)‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12(R)‐HETE), 12(S)‐HETE and leukotriene B 4 (LTB 4 ) for human mixed peripheral blood lymphocytes has been assessed in a 48‐well microchemotaxis assay. Responses to the standard lymphocyte chemoattractants, zymosan‐activated plasma, casein and N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) were also measured. 2 12(R)‐HETE was shown to be chemotactic for lymphocytes over the range 5 × 10 −7 to 5 × 10 −5 m . In contrast, negligible chemotactic responses to 12(S)‐HETE were obtained. 3 LTB 4 was 200 times more potent than 12(R)‐HETE as a lymphocyte chemoattractant, although maximal responses to the two agonists were similar. 4 12(R)‐HETE and LTB 4 , which are present in extracts of samples from the skin lesions of psoriasis, may be, at least in part, responsible for the lymphocyte infiltrate which is a characteristic feature of this disease.