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Amino acid pharmacology in neocortical slices: evidence for bimolecular actions from an extension of the Hill and Gaddum‐Schild equations
Author(s) -
Williams Thelma L.,
Smith D.A.S.,
Burton N.R.,
Stone T.W.
Publication year - 1988
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1988.tb11708.x
Subject(s) - quinolinic acid , kynurenic acid , schild regression , chemistry , antagonist , nmda receptor , 2 amino 5 phosphonovalerate , agonist , pyramidal cell , neuroscience , amino acid , stereochemistry , biophysics , receptor , biochemistry , tryptophan , biology , excitatory amino acid antagonists , hippocampal formation
1 The equivalent circuit of the cortical slice preparation has been analysed to show that the measured d.c. voltages can be used to estimate the Hill coefficient for ligands acting on the pyramidal cell bodies. 2 The Gaddum‐Schild equation for agonist‐antagonist interactions has been modified for applications in which the Hill coefficient is not equal to 1. 3 The equations obtained have been applied to recent data of Burton et al . (1988). The results provide evidence for a bimolecular action of the agonists N‐methyl‐D‐aspartate (NMDA) and quinolinic acid and the antagonists kynurenic acid and 2‐amino‐5‐phosphono‐pentanoic acid at NMDA receptors on mouse neocortical pyramidal cells.