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Effects of anterior hypothalamic lesions and sham‐operations on bacterial endotoxin‐induced non‐specific airway hyperreactivity in vivo and in vitro
Author(s) -
Oosterhout Antoon J.M.,
Nijnanten Felice M.A.,
Folkerts Gert,
Nijkamp Frans P.
Publication year - 1988
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1988.tb11603.x
Subject(s) - muscarinic acetylcholine receptor , in vivo , histamine , airway resistance , medicine , guinea pig , in vitro , endocrinology , acetylcholine , respiratory system , anesthesia , biology , pharmacology , receptor , biochemistry , microbiology and biotechnology
1 In the present study the effects of anterior hypothalamic (AHA) lesions and sham‐operations were investigated on the endotoxin‐induced airway hyperreactivity in guinea‐pigs. Unoperated, sham‐operated and AHA‐lesioned guinea‐pigs were injected intraperitoneally with Escherichia coli endotoxin and the airway reactivity tested four days later in isolated tracheal spirals and in spontaneously breathing anaesthetized animals. Control animals were given sterile saline. 2 Sham‐operated control animals demonstrated a diminished responsiveness of the tracheal spirals in vitro and of the lung resistance (ΔR 1 ) in vivo to histamine receptor and cholinoceptor‐muscarinic agonists as compared to unoperated control animals. 3 AHA‐lesioned control animals showed a responsiveness of the respiratory airways in vitro and in vivo between the values of unoperated and sham‐operated control animals, suggesting that lesions partially restored the diminished responsiveness. 4 In unoperated and sham‐operated guinea‐pigs, endotoxin administration induced hyperreactivity of the tracheal spirals and ΔR 1 to histamine receptor and cholinoceptor‐muscarinic agonists with respect to the control groups. 5 In AHA‐lesioned animals, the endotoxin‐induced airway hyperreactivity in vitro and in vivo to histamine receptor and cholinoceptor‐muscarinic agonists was absent.