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Effects of calcium channel blockade in canine saphenous veins after storage at −190°C
Author(s) -
Ebeigbe Anthony B.,
MüllerSchweinitzer Else,
Vogel Arnold
Publication year - 1988
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1988.tb11540.x
Subject(s) - diltiazem , nifedipine , verapamil , chemistry , dihydropyridine , calcium , calcium channel , gallopamil , fissipedia , pharmacology , medicine , organic chemistry
1 Canine saphenous veins were investigated in vitro either within 24 h after removal or after storage at −190°C for 4–5 weeks in foetal calf serum containing 1.8 m dimethyl sulphoxide. 2 Contractions and 45 Ca 2+ uptake in response to both depolarization and guanfacine were studied in the absence and presence of the calcium channel antagonists diltiazem, verapamil, nifedipine and the two stereoisomers of a 1,4‐dihydropyridine derivative, namely the (+)−(S) enantiomer and the (−)−( R ) enantiomer of 202–791 (isopropyl 4‐(2,1,3‐benzoxadiazol‐4‐yl)‐l,4‐dihydro‐2, 6‐dimethyl‐5‐nitro‐3‐pyridinecarboxylate). 3 Comparison of the data obtained on unfrozen and frozen/thawed veins revealed a good preservation of both contractile responsiveness and 45 Ca 2+ uptake mechanisms after storage at −190°C. 4 It is suggested that cryopreservation is a useful technique for storing venous smooth muscle for pharmacological studies.