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Effects of diuretics on GABA‐gated chloride current in frog isolated sensory neurones
Author(s) -
Inomata N.,
Ishihara T.,
Akaike N.
Publication year - 1988
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1988.tb10326.x
Subject(s) - amiloride , chemistry , furosemide , chloride channel , endocrinology , medicine , biophysics , pharmacology , chloride , reversal potential , patch clamp , sodium , biochemistry , receptor , biology , organic chemistry
1 Effects of three diuretics (furosemide, amiloride and α‐human atrial natriuretic polypeptide (α‐hANP)) on GABA‐activated chloride current (I Cl ) were investigated in frog isolated sensory neurones, following suppression of Na + , K + and Ca 2+ currents, by use of a ‘concentration‐clamp’ technique. 2 Furosemide inhibited the GABA‐activated I Cl in a non‐competitive manner and facilitated the inactivation phase, while amiloride inhibited the GABA response in a competitive manner, both inhibitions being concentration‐dependent. α‐hANP had no effects on the GABA‐induced I Cl . 3 The reversal potential of GABA‐activated I Cl (E GABA ) was not shifted in the presence of amiloride or furosemide. 4 The results suggest that amiloride may act at the GABA binding site while furosemide may act on the GABA‐gated chloride channel.