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Human calcitonin gene‐related peptide activates adenylate cyclase and releases prostacyclin from human umbilical vein endothelial cells
Author(s) -
Crossman D.,
McEwan J.,
MacDermot J.,
MacIntyre I.,
Dollery C.T.
Publication year - 1987
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1987.tb11373.x
Subject(s) - prostacyclin , cyclase , umbilical vein , bradykinin , medicine , endocrinology , calcitonin gene related peptide , adenylate kinase , chemistry , calcitonin , biology , in vitro , biochemistry , neuropeptide , receptor , stimulation
1 Endothelial cells of human umbilical vein were isolated and cultured in vitro.2 In these cells there was a concentration‐dependent release of prostacyclin and activation of adenylate cyclase by human calcitonin gene‐related peptide (hCGRP). The concentration of hCGRP for half‐maximum activation of adenylate cyclase ( K act ) by hCGRP was 190 n m . 3 Bradykinin induced a ten fold greater release of prostacyclin than CGRP, but did not activate adenylate cyclase. 4 hCGRP may exert its potent vasodilator properties by stimulating release of vasorelaxant substances, including prostacyclin from endothelial cells.