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Recovery of prostacyclin synthesis by rabbit aortic endothelium and other tissues after inhibition by aspirin
Author(s) -
Frazer C.E.,
Ritter J.M.
Publication year - 1987
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1987.tb09006.x
Subject(s) - prostacyclin , radioimmunoassay , endothelium , aspirin , aorta , thromboxane , cycloheximide , prostaglandin , cyclooxygenase , medicine , chemistry , endocrinology , thromboxane b2 , platelet , prostaglandin e , biochemistry , enzyme , protein biosynthesis
1 The effect of aspirin on prostacyclin (PGI 2 ) and thromboxane B 2 (TXB 2 ) synthesis was studied in rabbits. Tissues were removed from animals killed at intervals after injection of aspirin, and incubated with Hanks' solution. PGI 2 synthesis was monitored by radioimmunoassay of its hydrolysis product, 6‐ oxo‐prostaglandin F 1α (6‐oxo‐PGF 1α ). TXB 2 production in clotted blood, also measured by radioimmunoassay, was determined as an index of platelet cyclo‐oxygenase activity. 2 6‐oxo‐PGF 1α and TXB 2 production 0.5 h after aspirin were similarly inhibited to less than 5% of control in all incubations. Subsequent recovery of PGI 2 synthesis occurred more rapidly in aortic endothelium than in other tissues, including aorta denuded of endothelium. Recovery of TXB 2 production was slower than that of PGI 2 . 3 Intravenous cycloheximide prevented the partial recovery of PGI 2 synthesis that otherwise occurred 6 h after aspirin, while intravenous epidermal growth factor increased recovery. 4 It is concluded that in the rabbit, cyclo‐oxygenase is synthesized more rapidly in aortic endothelium than in deeper layers of aorta, or in the other tissues studied.