Premium
Calcitonin gene‐related peptide is localised to human airway nerves and potently constricts human airway smooth muscle
Author(s) -
Palmer James B.D.,
Cuss Francis M.C.,
Mulderry Peter K.,
Ghatei Mohamed A.,
Springall David R.,
Cadieux Alain,
Bloom Stephen R.,
Polak Julia M.,
Barnes Peter J.
Publication year - 1987
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1987.tb08987.x
Subject(s) - calcitonin gene related peptide , endocrinology , medicine , calcitonin , carbachol , tetrodotoxin , atropine , contraction (grammar) , radioimmunoassay , neuropeptide , chemistry , receptor
1 In human airways synthetic human sequence calcitonin gene‐related peptide (hCGRP), a novel peptide produced by alternative processing of mRNA from the calcitonin gene, caused concentration‐dependent contraction of human bronchi (EC 50 4.9 × 10 −9 m ) and was significantly more potent than substance P or carbachol. 2 The contractile response was unaffected by atropine (2 × 10 −6 m ), propranolol (10 −6 m ), indomethacin (10 −5 m ), tetrodotoxin (3 × 10 −6 m ), chlorpheniramine (10 −4 m ), cimetidine (10 −5 m ), or FPL55712 (10 −4 m ) suggesting a direct effect of CGRP on airways smooth muscle. 3 CGRP was detected in human airways by radioimmunoassay with highest concentrations in cartilaginous airways. 4 CGRP was localised by immunocytochemistry to both nerves and ganglia in human airways. 5 CGRP, is a potent constrictor of human airways and may have important effects on airway function and be implicated in the pathogenesis of bronchial hyper‐responsiveness and asthma.