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Cardiac hypoxia and subsequent reoxygenation: sensitivity to L‐arginine methylester
Author(s) -
Baccaro Cecilia,
Bennardini F.,
Dini Germana,
Franconi Flavia,
Giotti A.,
Matucci Rosanna,
Minuti Paola
Publication year - 1986
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1986.tb14581.x
Subject(s) - lactate dehydrogenase , arginine , ventricular fibrillation , hypoxia (environmental) , medicine , saline , basal (medicine) , guinea pig , endocrinology , chemistry , perfusion , anesthesia , enzyme , biochemistry , amino acid , oxygen , organic chemistry , insulin
1 The effect of L‐arginine methylester (L‐Arg‐Me) was studied in the isolated heart of the guinea‐pig perfused with hypoxic substrate‐free medium for 30 min and subsequently reoxygenated with normal saline solution for 30 min. 2 The administration of L‐Arg‐Me in basal conditions decreases dose‐dependently heart rate without any changes in the myocardial structure. 3 On the other hand, the administration of L‐Arg‐Me (5–10 m m ) decreases ventricular arrhythmias, especially during reoxygenation; in fact ventricular fibrillation is abolished. 4 L‐Arg‐Me treatment increases the recovery of normal electrical and mechanical activity at the end of reoxygenation and reduces the increase in basal tone. 5 Treatment with 10 m m L‐Arg‐Me decreases lactate dehydrogenase (LDH) release in the effluent and lysosomal fragility in cardiac tissue, while it does not influence calcium gain. 6 L‐Arginine (L‐Arg) does not mimic any of the effects of L‐Arg‐Me.