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A tachykinin antagonist inhibits gastric emptying and gastrointestinal transit in the rat
Author(s) -
Holzer P.,
HolzerPetsche U.,
Leander S.
Publication year - 1986
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1986.tb11144.x
Subject(s) - gastric emptying , substance p , medicine , bombesin , bethanechol , endocrinology , chemistry , neurokinin a , antagonist , motility , pharmacology , stomach , neuropeptide , muscarinic acetylcholine receptor , biology , receptor , genetics
1 The effect of a substance P antagonist, [D‐Pro 2 , D‐Trp 7,9 ]‐substance P (SPA), on gastric emptying and gastrointestinal transit in the rat was studied in order to elucidate a possible physiological role of endogenous substance P and other tachykinins in gastrointestinal motility. SPA was given by intraperitoneal injection concurrently with the intragastric administration of a test meal containing charcoal and 51 Cr. 2 Examination 15 min after the test meal showed that SPA (0.13‐1.3 μmol kg −1 ) inhibited gastric emptying and gastrointestinal transit in a dose‐dependent manner. 3 The inhibitory effect of SPA on gastric emptying and gastrointestinal transit remained unchanged after pretreatment of rats with mepyramine (8.7 μmol kg −1 ) plus cimetidine (19.8 μmol kg −1 ) or with guanethidine (67 μmol kg −1 ). 4 Since a full examination of SPA as a specific tachykinin antagonist was not possible in vivo , SPA was also tested on circular muscle strips from the rat gastric corpus in vitro. Submaximal contractions in response to bombesin or bethanechol were not reduced by SPA (50 μM), whereas those in response to substance P were inhibited. 5 The results suggest that SPA inhibits gastric emptying and gastrointestinal transit by interfering with the action of tachykinins released from enteric nerves and that endogenous tachykinins are involved in the regulation of gastrointestinal motility.