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Effects of opioid peptides and morphine on histamine‐induced catecholamine secretion from cultured, bovine adrenal chromaffin cells
Author(s) -
Livett Bruce G.,
Marley Philip D.
Publication year - 1986
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1986.tb10264.x
Subject(s) - catecholamine , histamine , adrenal medulla , endocrinology , secretion , chromaffin cell , medicine , opioid peptide , morphine , opioid , chemistry , receptor
1 The effect of opioid peptides and morphine on histamine‐induced catecholamine secretion has been studied in monolayer cultures of dispersed, bovine adrenal chromaffin cells. 2 Histamine‐induced a dose‐dependent secretion of both adrenaline and noradrenaline with a threshold dose of approximately 5 nM, an EC 50 of 150 nM and maximal secretion at 10 μM. 3 Catecholamine secretion induced by 1 μM histamine was completely dependent on extracellular calcium, was inhibited in a dose‐dependent manner by mepyramine (1 nM‐1 μM), and was unaffected by cimetidine (10 μM) and hexamethonium (0.1 mM). 4 Dynorphin‐1–13 (1 nM‐20μM), metorphamide (0.1 nM‐10μM), morphine (1 nM − 0.1 mM) and diprenorphine (1 nM − 0.1 mM) each had no effect on adrenaline or noradrenaline secretion induced by 1 μM histamine. 5 The characteristics of histamine‐induced catecholamine secretion from bovine adrenal chromaffin cells were similar to those reported previously for cat and rat adrenal medulla being calcium‐dependent and mediated by H 1 histamine‐receptors. The results with opioid peptides and morphine suggest that endogenous adrenal opioid peptides do not act on the opioid binding sites found on adrenal medullary chromaffin cells to modify their secretory response to histamine.