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A comparison of the acute inflammatory response in adrenalectomised and sham‐operated rats
Author(s) -
Flower R.J.,
Parente L.,
Persico Paola,
Salmon J.A.
Publication year - 1986
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1986.tb10156.x
Subject(s) - medicine , endocrinology , zymosan , leukotriene b4 , inflammation , prostaglandin , eicosanoid , corticosterone , in vivo , leukotriene , chemistry , phospholipase a2 , thromboxane , hormone , biology , in vitro , platelet , arachidonic acid , biochemistry , microbiology and biotechnology , asthma , enzyme
1 Carrageenin pleurisy was induced in adrenalectomised (ADX) and sham‐operated (SHO) rats. 2 The magnitude and duration of inflammation, as estimated by fluid exudation and cell migration, was greatly increased (approximately doubled) in ADX rats compared with that in their SHO controls. 3 The content of eicosanoids (6‐keto‐prostaglandin F 1α (6‐keto‐PGF 1α ), thromboxane B 2 (TXB 2 ), and leukotriene B 4 (LTB 4 )) in inflammatory exudates from ADX rats was significantly (2–4 fold) greater than that of their SHO controls. 4 Resident macrophages obtained from ADX rats produced more eicosanoids per cell per unit time when stimulated in vitro with zymosan, than did cells from the SHO controls. 5 Administration of glucocorticoids blocked the inflammatory response and reduced the release of eicosanoids both in vitro and in vivo in both groups of rats. 6 These data are consistent with the notion that physiological amounts of glucocorticoids exert a tonic inhibitory action on phospholipase activity in normal animals and that the increased secretion of these hormones during the inflammatory response serves to check and control the development of inflammation.