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Prostaglandin formation by isolated gastric parietal and nonparietal cells of the rat
Author(s) -
Postius S.,
Ruoff H. J.,
Szelenyi I.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb17381.x
Subject(s) - arachidonic acid , prostaglandin e , percoll , histamine , parietal cell , chemistry , gastric mucosa , prostaglandin , medicine , endocrinology , biochemistry , biology , stomach , in vitro , enzyme
1 Rat gastric cells isolated by pronase and subdivided by Percoll into 3 fractions (F1, F2, F3) were used to study prostaglandin E 2 (PGE 2 ) formation and, as an indirect measure of parietal cell H + production, [ 14 C]‐aminopyrine uptake. 2 Cells that had not been fractionated, with 20 to 25% parietal cells, contained at 0°C 1.7 ± 0.35 (s.e.mean) ng PGE 2 10 8 cells −1 . During incubation at 37°C these cells steadily synthesized up to 4.36 ± 0.73 ng PGE 2 10 8 cells −1 from endogenous substrate. 3 Indomethacin in concentrations higher than 10 −6 moll −1 inhibited this basal formation completely, but 10 −4 moll −1 did not reduce the cellular PGE 2 level below 1.4 ± 0.2 ng 10 8 cells −1 . 4 Arachidonic acid in concentrations higher than 10 −5 moll −1 evoked an abundant formation of PGE 2 , and 10 −4 moll −1 built up a plateau of over 7.5 ± 1.65 ng PGE 2 10 8 cells −1 within 15 min. 5 PGE 2 formation in cell fractions increased significantly with the number of parietal cells per assay tube. 6 Indomethacin (10 −8 to 10 −4 moll −1 ) did not influence the histamine‐stimulated uptake of [ 14 C]‐aminopyrine, while arachidonic acid (10 −5 to 10 −4 moll −1 ) inhibited this process. 7 PGE 2 formation in response to arachidonic acid was prevented by indomethacin, but the inhibition of aminopyrine uptake by arachidonic acid could not be prevented by indomethacin. 8 The data suggest that isolated gastric cells of the rat sustain constant PGE 2 synthesis in vitro , which is more pronounced in parietal than in mucosal and chief cells. PGE 2 may exert different effects within distinct gastric cell types.