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Identification of presynaptic β 2 ‐adrenoceptors on the sympathetic nerve fibres of the human pulmonary artery
Author(s) -
Göthert Manfred,
Hentrich Frank
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb11094.x
Subject(s) - rauwolscine , isoprenaline , atenolol , agonist , chemistry , medicine , endocrinology , prazosin , propranolol , pulmonary artery , fenoterol , antagonist , receptor , blood pressure , stimulation , asthma
1 Strips of human pulmonary arteries from patients undergoing surgery for lung tumour were incubated with [ 3 H]‐noradrenaline. Subsequently, they were supervised with physiological salt solution containing cocaine and corticosterone. Tritium overflow from the strips was stimulated by transmural electrical impulses (2 Hz). The electrically evoked overflow of tritium consisted of 91% unmetabolized [ 3 H]‐noradrenaline, and this percentage was not altered by isoprenaline. 2 Adrenaline (in the presence of rauwolscine), isoprenaline and the preferential β 2 ‐adrenoceptor agonist, procaterol, concentration‐dependently increased the electrically evoked tritium overflow. Prenalterol, a β‐adrenoceptor agonist with moderate preference for β,‐adrenoceptors, was considerably less active than the previously mentioned agonists; noradrenaline (in the presence of rauwolscine) was ineffective. 3 The concentration‐response curve of procaterol was shifted to the right by the preferential β 2 ‐adrenoceptor antagonist ICI 118–551 but was not affected by the β 1 ‐selective antagonist, atenolol. Propranolol, but not atenolol, produced a shift to the right of the concentration‐response curve of isoprenaline. 4 It is concluded that the sympathetic nerve fibres of the human pulmonary artery are endowed with facilitatory presynaptic β 2 ‐adrenoceptors.