Premium
Inhibition of ionophore‐stimulated leukotriene B 4 production in human leucocytes by monohydroxy fatty acids
Author(s) -
Camp R.D.R.,
Fincham N.J.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb11082.x
Subject(s) - ionophore , chemistry , leukotriene , biochemistry , leukotriene b4 , immunology , biology , membrane , inflammation , asthma
1 Leukotriene B 4 (LTB 4 ) release by calcium ionophore‐stimulated human leucocytes was measured by use of selective solvent partition of reaction mixtures and an agarose microdroplet chemokinesis assay, and the inhibitory effects of four monohydroxy fatty acids were determined. 2 15‐Hydroxy‐eicosatetraenoic acid (15‐HETE) was the most effective inhibitor of LTB 4 production with an approximate IC 50 value of 6 μM and 99% inhibition at 50 μM, whereas 13‐hydroxyoctadecadienoic acid (13‐HODD) and 12‐HETE were weaker inhibitors with approximate IC 50 values of 32 μM and 23 μM, and 59% and 68% inhibition at 50 μM, respectively. 3 We suggest that 13‐HODD and 12‐HETE, which are present in large amounts in the lesions of the skin disease psoriasis, may act as endogenous modulators of 5‐lipoxygenase activity in skin.