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Transmembrane sodium and potassium gradients modulate histamine secretion induced by ionophore A23187
Author(s) -
Amellal M.,
Bronner C.,
Landry Y.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb11080.x
Subject(s) - ionophore , calcium , histamine , extracellular , potassium , chemistry , ouabain , sodium , mast cell , secretion , egta , biochemistry , endocrinology , medicine , biophysics , biology , immunology , organic chemistry
1 Histamine secretion was induced from rat peritoneal mast cells by calcium ionophore A23187 in the presence of various extracellular calcium concentrations. Transmembrane sodium and potassium gradients were altered by cold pretreatment of mast cells or through the inhibition of sodium‐potassium ATPase by the use of ouabain or potassium‐deprivation. 2 Such pretreatments led to a parallel shift to the left of the extracellular calcium concentration‐histamine secretion curve, i.e. to an apparent decrease of extracellular calcium requirement for the ionophore‐induced histamine release. 3 These effects were fully reversed by warming mast cells, by washing out ouabain or by adding potassium. Metabolic inhibition of mast cells prevented the ionophore‐induced secretion in all the experimental conditions described. Secretion observed in the absence of added calcium was inhibited by short term treatment of cells with 5 times 10 −6 M EGTA or EDTA provided magnesium was absent from the assay medium. 4 Data show that ionophore A23187 was able to induce secretion in the presence of micromolar concentrations of extracellular calcium, when the efficiency of the ionophore was not decreased by extracellular magnesium and when transmembrane sodium and potassium gradients were altered.