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[ 3 H]‐tetracaine binding on rat synaptosomes and sodium channels
Author(s) -
Grima Michèle,
Schwartz Jean,
Spach MarieOdile,
Velly Jeanne
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb09442.x
Subject(s) - tetracaine , procaine , chemistry , binding site , biophysics , sodium channel , sodium , pharmacology , biochemistry , anesthesia , lidocaine , biology , medicine , organic chemistry
1 [ 3 H]‐tetracaine binding was studied in a rat synaptosomal preparation. [ 3 H]‐tetracaine bound to a single class of binding sites with a mean K D of 188 ± 28 nM and a mean maximal binding capacity of 13 ± 0.7 pmol mg −1 protein. 2 [ 3 H]‐tetracaine binding was inhibited by tetracaine, procaine and by β‐adrenoceptor blocking agents which possess local anaesthetic properties. 3 [ 3 H]‐tetracaine binding was not modified by neurotoxins interacting specifically with the sodium channels.