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Increased metabolism of arachidonic acid in an immune model of colitis in guinea‐pigs
Author(s) -
BoughtonSmith N.K.,
Whittle B.J.R.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb08913.x
Subject(s) - arachidonic acid , metabolism , thromboxane b2 , prostaglandin e2 , guinea pig , metabolite , chemistry , immune system , thromboxane a2 , colitis , prostaglandin , endocrinology , prostaglandin e , inflammation , medicine , pharmacology , biochemistry , biology , immunology , enzyme , receptor , platelet
1 Inflammation of the guinea‐pig colon was produced by skin sensitization and subsequent intracolonic challenge with the chemical hapten, dinitrochlorobenzene. 2 Metabolism of [ 14 C]‐arachidonic acid by homogenates of control colon was very low, although metabolites co‐migrating on thin layer chromatography (t.l.c.) with prostaglandin E 2 (PGE 2 ), PGF 2α , PGD 2 , 6‐keto‐PGF 1α , thromboxane B 2 (TXB 2 ), HHT and 11‐, 12‐, 15‐HETE were formed. 3 There was an overall 3 fold increase in metabolism of [ 14 C]‐arachidonic acid by homogenates of inflamed mucosa. The greatest increase in metabolite formation was of PGE 2 , with smaller increases in HHT, 11‐, 12‐, 15‐HETE, PGD 2 , TXB 2 , PGF 2α and 6‐keto‐PGF 1α . The formation of these metabolites was inhibited both by indomethacin and the dual pathway inhibitor, BW755C. 4 The formation of immunoreactive PGE 2 , TXB 2 and 6‐keto‐PGF 1α was also increased in homogenates of inflamed guinea‐pig colon. The small level of immunoreactive LTB 4 detected in control colon was not changed in inflamed colonic tissue. 5 The dinitrochlorobenzene model of colitis offers a means of studying arachidonic acid metabolism in an immune‐mediated inflammatory response in intestinal tissue.