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Interaction of prostaglandin D 2 with prostacyclin, carbacyclin and the hydantoin prostaglandin, BW245C, in guinea‐pig platelets
Author(s) -
Hamid S.,
Whittle B.J.R.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb08858.x
Subject(s) - prostacyclin , guinea pig , platelet , prostaglandin , chemistry , antagonism , medicine , pharmacology , inhibitory postsynaptic potential , forskolin , endocrinology , in vitro , biology , biochemistry , receptor
1 The anti‐aggregating actions of prostaglandin D 2 (PGD 2 ) have been compared to prostacyclin (PGI 2 ), its stable analogue carbacyclin and a hydantoin prostaglandin, BW245C, in guinea‐pig platelets in vitro . 2 PGI 2 , carbacyclin and BW245C were potent inhibitors of ADP‐induced aggregation in guinea‐pig platelets, with ID 50 values comparable to those obtained in human platelet‐rich‐plasma. 3 In contrast, PGD 2 acted as a weak and partial inhibitor in guinea‐pig platelet aggregation, producing a bell‐shaped dose‐response relationship. 4 PGD 2 induced a dose‐related antagonism of the inhibitory actions of BW245C, prostacyclin and carbacyclin on guinea‐pig platelets. 5 However, PGD 2 did not antagonize the inhibitory actions of either forskolin or dibutyryl cyclic AMP on this platelet preparation. 6 The results suggest a non‐specific interaction of PGD 2 with these prostanoid binding sites on guinea‐pig platelets.