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Adenosine potentiates immunological histamine release from rat mast cells by a novel cyclic AMP‐independent cell‐surface action
Author(s) -
Churchi Martin K.,
Hughes Philip J.
Publication year - 1985
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1985.tb08823.x
Subject(s) - adenosine , histamine , theophylline , mast cell , adenylate kinase , cyclase , chemistry , endocrinology , adenosine monophosphate , medicine , histamine h2 receptor , purinergic receptor , purinergic signalling , pharmacology , adenosine receptor , biology , biochemistry , immunology , enzyme , receptor , agonist , antagonist
Adenosine enhanced histamine release and prolonged the adenosine 3′:5′‐cyclic monophosphate (cyclic AMP) response in purified rat peritoneal mast cells following immunological challenge. The effect on the cyclic AMP response, which was blocked by 8‐phenyltheophylline, probably results from an interaction with A 2 ‐purinoceptors. Enhancement of histamine release showed different characteristics. It was not inhibited by dipyridamole or hexobendine, thereby indicating an action at the cell surface. However, the relative potencies of adenosine analogues and nucleotides, together with the observation that this effect was not antagonized by 8‐phenyltheophylline or theophylline, suggest that it is not mediated by a previously recognised purinoceptor. Thus, enhancement of histamine release may represent a novel cell surface action of adenosine which is independent of its effects on adenylate cyclase.