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Location of [ 125 I]‐angiotensin II receptors on rat kidney cortex epithelial cells
Author(s) -
Cox Helen M.,
Munday Kenneth A.,
Poat Judith A.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb16487.x
Subject(s) - brush border , angiotensin ii , renal cortex , receptor , membrane , medicine , endocrinology , chemistry , kidney , cortex (anatomy) , renin–angiotensin system , biology , biophysics , microbiology and biotechnology , biochemistry , vesicle , neuroscience , blood pressure
1 Angiotensin II (AII) stimulates active Na + extrusion from Na + loaded renal cortex slices. 2 Specific high affinity [ 125 I]‐AII binding sites in partially purified basolateral and brush‐border epithelial membranes exhibit a K D of 0.88 n M and B max of 321.13 fmol mg −1 protein. 3 Separation and purification of brush‐border membranes yielded high affinity [ 125 I]‐AII binding sites with K D of 1.02 n M and B max of 56.6 fmol mg −1 protein. 4 Angiotensin II receptors of the same affinity are present on renal cortex brush‐border and basolateral membranes but a greater proportion are located on the latter. These receptors may be involved in the direct control of Na + and water transport by AII.