Premium
Effect of chemical destruction of adrenergic neurones on some cholinergic mechanisms in adult rat sympathetic ganglia
Author(s) -
Collier B.,
Johnson G.,
Quik M.,
Welner S.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb16479.x
Subject(s) - adrenergic , cholinergic , sympathetic nervous system , sympatholytics , neuroscience , norepinephrine , chemistry , endocrinology , biology , medicine , dopamine , receptor , blood pressure
1 Rats were treated for 2–6 weeks with guanethidine after which their superior cervical ganglia were removed. 2 Ganglionic tyrosine hydroxylase and α‐bungarotoxin binding sites were reduced by the guanethidine treatment indicating adrenergic cell body destruction. 3 Choline acetyltransferase activity and acetylcholine content of ganglia were not clearly changed by the guanethidine treatment, indicating that the drug does not destroy presynaptic terminals and that these presynaptic indicators do not adapt markedly to postsynaptic loss. 4 The Cholinesterase in the ganglia was reduced by guanethidine treatment, but such ganglia retained their ability to accumulate surplus acetylcholine when they were incubated with physostigmine. This is interpreted as indicating surplus acetylcholine accumulation is a presynaptic phenomenon. 5 Choline uptake by resting ganglia was not reduced as a result of guanethidine treatment nor was it affected by preganglionic denervation. This is interpreted as indicating that during rest, choline uptake is into supporting cells or intraganglionic cells rather than cholinergic nerve terminals or adrenergic cell bodies.