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Release of kallikrein‐like esterase and tonin from dispersed cells of the rat submandibular gland
Author(s) -
Izumi Hiroshi
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb16456.x
Subject(s) - kallikrein , submandibular gland , esterase , endocrinology , isoprenaline , medicine , chemistry , agonist , phenoxybenzamine , stimulation , receptor , biology , biochemistry , propranolol , enzyme
1 Viable dispersed cell preparations of rat submandibular gland were obtained by a tissue‐dissociation procedure using collagenase and gentle mechanical force. 2 The cells released kallikrein‐like esterase in a time‐ and calcium‐dependent manner in response to noradrenaline (10 μ M ) at 30°C. The net loss of kallikrein‐like esterase content from the dispersed cells corresponded with the increase in kallikrein‐like esterase activity in the suspending medium at all concentrations of noradrenaline. These results indicate the viability and functional integrity of this dispersed cell preparation of rat submandibular gland. 3 α‐Adrenoceptor agonists such as noradrenaline stimulated kallikrein‐like esterase and tonin release in a dose‐dependent manner, whereas the β‐adrenoceptor agonist isoprenaline and cholinoceptor agonist methacoline were both inactive. Noradrenaline‐induced release of kallikrein‐like esterase and tonin were completely blocked by prior addition of the α‐adrenoceptor antagonist, phenoxybenzamine. 4 It is concluded that the secretion of kallikrein‐like esterase and tonin in rat submandibular gland is mediated only via stimulation of α‐adrenoceptors.