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Differential effects of prostaglandin E 2 and cyclic AMP on release of arachidonic acid metabolites from resting and lipopolysaccharide‐stimulated macrophages
Author(s) -
Feuerstein Nili,
Ramwell Peter W.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb16230.x
Subject(s) - thromboxane , prostaglandin e , arachidonic acid , medicine , endocrinology , thromboxane b2 , thromboxane a2 , adenosine , chemistry , prostaglandin , bucladesine , lipopolysaccharide , biology , biochemistry , platelet , enzyme , stimulation
1 The present study investigated the effect of cyclic adenosine 3′,5′‐monophosphate (cyclic AMP) and prostaglandin E 2 (PGE 2 ) on arachidonic acid metabolism in rat peritoneal macrophages. 2 Dibutyryl cyclic AMP (db‐cyclic AMP) caused differential effects on the synthesis of PGE 2 and thromboxane. Although db cyclic AMP enhanced the release of PGE 2 , it inhibited the release of thromboxane. This suggests that cyclic AMP may regulate cellular functions via induction of a shift in the proportion of arachidonic acid metabolites. 3 PGE 2 , at low concentrations, markedly inhibited thromboxane release in nontreated macrophages, but it had virtually no effect on thromboxane release in cells treated with lipopolysac‐charide (LPS). By contrast, db‐cyclic AMP inhibited thromboxane release also in LPS‐stimulated cells. 4 The interrelationships between PGE 2 , thromboxane and cyclic AMP, and possible interference of LPS in these interactions are discussed.