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Effects of neomycin on galactose absorption across rat jejunum
Author(s) -
Debnam E.S.,
Thompson C.S.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb10806.x
Subject(s) - neomycin , jejunum , galactose , absorption (acoustics) , chemistry , biochemistry , pharmacology , biology , antibiotics , materials science , composite material
1 The effects of neomycin sulphate on galactose absorption have been studied using in vivo and in vitro preparations of rat small intestine. 2 Neomycin (10 −3 m ) produced an increase in the maximum transport capacity ( J max ) for the active component of absorption in vivo . The apparent K t for absorption was unaffected. 3 The antibiotic caused a dose‐dependent increase in the potential difference across the mucosal membrane ( V m ) measured in vitro , a maximal effect being seen at a concentration of 10 −4 m . Furthermore, the magnitude of the depolarization induced by the addition of galactose (4 m m ) to the mucosal fluid was enhanced by neomycin (10 −4 m ). Phlorhizin (10 −4 m ) abolished the galactose‐induced depolarization in both the absence and presence of the antibiotic. 4 It is concluded that neomycin increases the electrical driving force for Na + during Na+‐coupled galactose entry into the enterocyte.