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Sodium‐potassium ATPase inhibition potentiates compound 48/80‐induced histamine secretion from mast cells
Author(s) -
Amellal M.,
Binck M.,
Frossard N.,
Ilien B.,
Landry Y.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb10777.x
Subject(s) - histamine , ouabain , potassium , calcium , chemistry , mast cell , egta , endocrinology , medicine , sodium , atpase , compound 48/80 , extracellular , pharmacology , biochemistry , biology , enzyme , immunology , degranulation , receptor , organic chemistry
1 The effect of ouabain on the histamine secretion induced by compound 48/80 has been studied using rat peritoneal mast cells. 2 Ouabain did not modify histamine release in the presence of millimolar concentrations of extracellular calcium. 3 However, when mast cells were previously washed with a calcium‐free buffer, ouabain strongly potentiated histamine release elicited by compound 48/80. 4 The full potentiation of mast cell secretion by ouabain required 30 min preincubation before adding compound 48/80. It was inhibited by lanthanum and EGTA. 5 Potassium deprivation mimicked the effect of ouabain. A 30 min preincubation time without potassium was also required. 6 Potassium concentrations below 2.7 m m increased the effect of ouabain whereas higher potassium concentrations reversed this effect. 7 The potentiation of compound 48/80‐induced histamine release by ouabain or potassium deprivation was not immediately reversed by washing away ouabain or by adding potassium, respectively. 8 The data confirm that sodium‐potassium ATPase is involved, through a calcium‐dependent process, in the regulation of histamine release from mast cells.