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A new in vivo method for the measurement of repetitive anaphylactic responses in the guinea‐pig
Author(s) -
Bottomley Kevin M.K.,
Lewis Stevan,
Rising Trevor J.,
Steward Alan
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb10761.x
Subject(s) - mepyramine , ovalbumin , sensitization , guinea pig , anaphylaxis , pharmacology , medicine , theophylline , anaphylactic shock , immunology , anesthesia , antigen , allergy , antagonist , receptor
1 The established Konzett‐Rossler bronchorespiratory model has been combined with a unique ovalbumin sensitization procedure to give a novel method to measure anaphylaxis in the anaesthetized guinea‐pig. 2 Following antigen challenge, up to eight equal bronchoconstrictor responses to the same dose can be generated from a single animal over a 120 min period. 3 Total inhibition of the anaphylactic response can be elicited by four different classes of compound, namely salbutamol, mepyramine, theophylline and dimaprit. Cromoglycate failed to cause any inhibition. 4 The method is discussed with particular reference to the antigen sensitization procedure, which differs substantially from other regimens previously employed and gives rise to heat labile antibody.