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The regulation of δ‐opiate receptor density on 108CC15 neuroblastoma × glioma hybrid cells
Author(s) -
Moses Margaret A.,
Snell Christopher R.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb10757.x
Subject(s) - opiate , neuroblastoma , receptor , enkephalin , endocrinology , medicine , adenosine , chemistry , endorphins , cell culture , glioma , morphine , in vitro , pharmacology , biology , opioid , biochemistry , cancer research , genetics
1 The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma × glioma hybrid cells has been studied. 2 Cell differentiation by culture in the presence of N 6 ‐O 2 ‐dibutyryl adenosine 3′,5′‐cyclic monophosphate induced a three fold increase in opiate receptor density. 3 When the cells were grown in the presence of 10 −5 m morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls. 4 Culture in the presence of 10 −7 md ‐Ala 2 ‐ d ‐Leu 5 ‐enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment. 5 The down regulation process could be inhibited by continued exposure to d ‐Ala 2 d ‐Leu 5 ‐enkephalin at concentrations greater than 4 n m ; below this concentration down regulation was rapid and irreversible. 6 A model to explain these observations is described.