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Influence of plasma protein on the inhibitory effects of indocyanine green and bromcresol green on pulmonary prostaglandin E 1 extraction
Author(s) -
Dawson Christopher A.,
Linehan John H.,
Rickaby David A.,
Roerig David L.
Publication year - 1984
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1984.tb10097.x
Subject(s) - indocyanine green , chemistry , extraction (chemistry) , prostaglandin , inhibitory postsynaptic potential , biophysics , chromatography , biochemistry , pharmacology , medicine , biology , pathology
1 The purpose of this study was to examine the influence of plasma protein on the inhibitory effects of the anionic dyes indocyanine green and bromcresol green on prostaglandin E 1 (PGE 1 ) uptake by the lungs. 2 Dog lung lobes were isolated and perfused with either autologous plasma or Krebs‐Ringer bicarbonate solution (KRB) containing no protein but with dextran used as a colloid. 3 PGE 1 uptake was determined by injecting a bolus, containing radiolabeled PGE 1 into the lobar artery and then analysing ethanolic extracts of the venous effluent for radioactivity in PGE 1 and PGE 1 metabolites by thin layer chromatography and scintillation counting. 4 When the lobes were perfused with KRB, bromcresol green at an average initial concentration of 28.5 μ m , reduced PGE 1 by an average of 56%. When the lobes were perfused with plasma, similar concentrations of bromcresol green reduced the uptake by less than 2%. 5 A similar result was obtained with indocyanine green, which at an average initial concentration of 17.5 μ m reduced uptake by about 70% when the lobes were perfused with KRB, but when the lobes were perfused with plasma similar concentrations of the dye reduced uptake by less than 3.5%. 6 The results suggest that plasma protein binding interferes with the inhibitory effects of these dyes on PGE 1 uptake in the lungs.