Premium
Effect of inhibition of synthesis and receptor antagonism of SRS‐ A in cardiac anaphylaxis
Author(s) -
Aehringhaus U.,
Peskar B.A.,
Wittenberg H.R.,
Wölbling R.H.
Publication year - 1983
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1983.tb11051.x
Subject(s) - nordihydroguaiaretic acid , leukotriene , anaphylaxis , guinea pig , thromboxane b2 , pharmacology , antagonist , prostaglandin , medicine , chemistry , lipoxygenase , endocrinology , receptor , biochemistry , immunology , allergy , platelet , enzyme , asthma
1 The effects of infusions of the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, 1.1 × 10 −7 mol min −1 ) and the antagonist of slow‐reacting substance of anaphylaxis (SRS‐A) FPL 55712 (1.2 × 10 −7 molmin −1 ) on the coronary constriction and the release of SRS‐A, leukotriene C 4 ‐like immunoreactivity, thromboxane B 2 and 6‐keto‐prostaglandin F 1α from perfused anaphylactic guinea‐pig hearts were investigated. 2 Both NDGA and FPL 55712 in the concentrations used induced an increase in basal coronary flow, but did not prevent the coronary flow reduction in the early phase (0–4 min) after antigen injection. On the other hand, NDGA and FPL 55712 inhibited the less pronounced long‐lasting coronary flow reduction in the later phase of cardiac anaphylaxis. 3 NDGA decreased the release of SRS‐A from the anaphylactic guinea‐pig hearts below or close to the detection limit of the bioassay and simultaneously diminished the release of leukotriene C 4 ‐like immunoreactivity. On the other hand, FPL 55712 did not influence the amounts of leukotriene C 4 ‐like immunoreactivity released in cardiac anaphylaxis. 4 Neither NDGA nor FPL 55712 affected the release of immunoreactive thromboxane B 2 (TXB 2 ) from anaphylactic guinea‐pig hearts. Release of 6‐keto‐prostaglandin F 1α after challenge, however, was decreased by NDGA, while FPL 55712 had no significant effect. 5 These results suggest, that SRS‐A may be a relatively more important mediator in the late phase of coronary constriction occurring during cardiac anaphylaxis, while the effects of other mediators, particularly vasoconstrictor cyclo‐oxygenase products, seem to prevail in the early phase.