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Differential anatomical location of [ 3 H]‐N, n ‐propylnorapomorphine and [ 3 H]‐spiperone binding sites in the striatum and substantia nigra of the rat
Author(s) -
Hall M.D.,
Jenner P.,
Kelly E.,
Marsden C.D.
Publication year - 1983
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1983.tb11035.x
Subject(s) - substantia nigra , striatum , dopamine , spiperone , medial forebrain bundle , chemistry , kainic acid , neuroscience , nucleus accumbens , pars reticulata , biology , dopamine receptor , biochemistry , dopaminergic , glutamate receptor , receptor
1 Specific [ 3 H]‐spiperone and [ 3 H]‐N, n ‐propylnorapomorphine (NPA) binding was measured in striatum and substantia nigra of the rat following unilateral 6‐hydroxydopamine (6‐OHDA) lesions of the medial forebrain bundle, kainic acid lesions of the substantia nigra or striatum, and following decortication. 2 Binding sites labelled by [ 3 H]‐spiperone in striatum were found to lie on striatal cell bodies and on the terminals of cortico‐striate glutamate fibres, but not on presynaptic dopamine terminals. In contrast, binding sites labelled by [ 3 H]‐NPA were demonstrated on striatal cell bodies and on the terminals of nigro‐striatal dopamine fibres, but not on cortical afferents. 3 In substantia nigra, specific [ 3 H]‐spiperone binding sites were found only on non‐dopamine cell bodies. No clear evidence was found for their existence on dopamine cell bodies, the terminals of strio‐nigral fibres or the terminals of cortico‐nigral fibres. In contrast, specific binding sites for [ 3 H]‐NPA were found on dopamine cell bodies and the terminals of strio‐nigral fibres. Localization on non‐dopamine cell bodies or on cortico‐nigral fibres was not demonstrated. 4 These studies support the concept of differential localization of agonist and antagonist binding sites.