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The pharmacological modulation of [ 3 H]‐disaturated phosphatidylcholine overflow from perifused lung slices of adult rats: a new method for the study of lung surfactant secretion
Author(s) -
Gilfillan A.M.,
Hollingsworth M.,
Jones A.W.
Publication year - 1983
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1983.tb11008.x
Subject(s) - propranolol , pulmonary surfactant , phosphatidylcholine , chemistry , endocrinology , lung , medicine , choline , atropine , choline chloride , potassium , dihydroalprenolol , chloride , sodium , biology , biochemistry , phospholipid , receptor , organic chemistry , membrane , partial agonist , antagonist
1 Lung slices from adult rats incubated in [methyl‐ 3 H]‐choline chloride formed [ 3 H]‐disaturated phosphatidylcholine ([ 3 H]‐DSPC) which was used as an index of lung surfactant. 2 The slices were perifused after 3 h incubation in [methyl‐ 3 H]‐choline chloride and the overflow of [ 3 H]‐DSPC, as a rate coefficient, was used as a measure of surfactant secretion. The basal overflow of [ 3 H]‐DSPC rapidly declined over the first 30 min of perifusion and then declined slowly. 3 Salbutamol induced a prolonged, and sometimes delayed, increase in [ 3 H]‐DSPC overflow, which was reduced by (±)‐propranolol. Potassium chloride produced an immediate, and usually transient, increase in [ 3 H]‐DSPC overflow which was not modified by atropine or (±)‐propranolol. Adenosine 5′‐triphosphate, but not phenylephrine, also increased [ 3 H]‐DSPC overflow. 4 This method can measure the magnitude and time‐course of lung surfactant secretion induced by drugs.