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Effect of 6‐hydroxydopamine on bovine adrenal chromaffin cells in culture
Author(s) -
Kirpekar S.M.,
Nobiletti J.,
Trifaró J.M.
Publication year - 1983
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1983.tb10540.x
Subject(s) - hydroxydopamine , chromaffin cell , catecholamine , toxicity , endocrinology , medicine , cell culture , biology , adrenal medulla , chemistry , dopamine , genetics , dopaminergic
1 The effect of 6‐hydroxydopamine on the catecholamine content and cell morphology of bovine adrenal chromaffin cells in culture was investigated. 2 6‐Hydroxydopamine markedly reduced the catecholamine content of the cultured chromaffin cells after 6 and 24 h exposure. The effect was dose‐related, with half‐maximal depletion occurring at 2.4 × 10 −5 M3 Cells exposed to 6‐hydroxydopamine for 3 h and then to normal medium without the drug for 3 h more showed the same degree of toxicity as cells exposed to the drug for the entire 6 h. 4 Ascorbate at high concentrations also exhibited toxicity toward chromaffin cells between 6 and 24 h of exposure. 5 6‐Hydroxydopamine produced marked changes in cell morphology. At 1 h the cells appeared normal, at 3 h the processes were markedly shortened, and at 6 h they were completely retracted. On exposure for 24 h there were gross morphological changes and most cells were detached and free‐floating in the medium. 6 The toxicity of 6‐hydroxydopamine in bovine adrenal chromaffin cells is discussed.