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Characterization and partial purification of ‘renocortins’: two polypeptides formed in renal cells causing the anti‐phospholipase‐like action of glucocorticoids
Author(s) -
Cloix Jean François,
Colard Odile,
Rothhut Bernard,
RussoMarie Françoise
Publication year - 1983
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1983.tb10526.x
Subject(s) - phospholipase a2 , isoelectric point , arachidonic acid , chromatofocusing , in vitro , phospholipase , phospholipase a , secretion , phospholipid , biochemistry , prostaglandin , chemistry , medicine , endocrinology , biology , enzyme , membrane
1 Anti‐inflammatory steroids reduce prostaglandin E 2 (PGE 2 ) synthesis in rat renomedullary interstitial cells in culture by inhibiting the release of arachidonic acid from membranous phospholipid stores, exhibiting antiphospholipase‐like properties. 2 After treatment of the cells with dexamethasone 10 −6 M , these cells release a protein in the supernatant. 3 This supernatant is able to inhibit PGE 2 secretion in untreated cells and to inhibit phospholipase A 2 activity in an in vitro system. 4 Using chromatofocusing separation, we showed that two distinct proteins exist with isoelectric points of 5.8 and 8.3. 5 Using gel permeation separation, we showed that two proteins exist with apparent molecular weights of 15,000 and 30,000 daltons. 6 We conclude that, in renal cells in culture, anti‐inflammatory steroids induce the synthesis and the release of two polypeptides which we have named ‘Renocortins’ (induced by corticoids in renal cells) causing the antiphospholipase‐like action of glucocorticoids. 7 Our results are in good agreement with others, but as renal cells are not directly involved in the inflammatory process, we suggest that this steroid‐induced phenomenon is not solely involved in the inflammatory reaction but is of more general physiological relevance.